文昌鸡性成熟启动前后下丘脑c-Myc/LIN28B/let-7a通路表达规律分析

doi:10.11843/j.issn.0366-6964.2016.04.010

Abstract

Abstract:

This experiment was conducted to investigate the role of c-Myc/LIN28B/let-7a pathway in chicken puberty onset.The Wenchang chicken breed was used as the material.The comb size，ovary weight，oviduct length and follicle size for hens were measured from 5 to 17 weeks.qRT-PCR was performed to analyze the hypothalalmic expression changes of c-Myc/LIN28B/let-7a pathways.The results showed that：1) The ovary and oviduct developed slowly and the follicles kept primitive in early period of development in Wenchang chicken.This period would last about 12 weeks.Then，the gonad tissues developed explosively，the ovary weight and oviduct length of 13 weeks increased 125% and 120% respectively compared to that of 12 weeks，and accompanied by emergence of pre-hierarchical follicles.Based on these，the crucial “timing” for transition from juvenile to puberty onset for Wenchang hens was determined as age of 13 weeks.2) qRT-PCR test revealed the relative expression levels of c-Myc and LIN28B genes in hypothalamus decreased significantly (P<0.05，P<0.01) at the transition timing of puberty onset in Wenchang chicken.The lower level of c-Myc could last to the age of first laying，but that of LIN28B recovered to higher level before the age of first laying.Accompanied with these changes was the significant increase of let-7a (P<0.01)，which could kept higher until the age of first laying.These results indicated that c-Myc→LIN28B┤let-7a regulating pathway was involved in chicken puberty onset.

CLC Number:

S831.2

HAN Wei，SU Yi-jun，ZHU Yun-fen，LI Guo-hui，ZHANG Hui-yong，YIN Jian-mei，WANG Ke-hua，ZOU Jian-min. Analysis on Changes of Hypothalamic c-Myc/LIN28B/let-7a Pathway Transcription during Puberty Onset in Wenchang Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 709-715.

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Analysis on Changes of Hypothalamic c-Myc/LIN28B/let-7a Pathway Transcription during Puberty Onset in Wenchang Chicken

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